文章基本信息

标题：VIABILITY AND PLASMA MEMBRANE INTEGRITY OF THE SPOTTED BUFFALO EPIDIDYMAL SPERMATOZOA AFTER THAWING WITH THE ADDITION OF DEXTROSE INTO THE EXTENDER
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作者：YULNAWATI YULNAWATI ; H. MAHESHWARI ; HERDIS 等
期刊名称：BIOTROPIA
印刷版ISSN：1907-770X
出版年度：2009
卷号：16
期号：1
DOI：10.11598/btb.2009.16.1.63
语种：English
出版社：SEAMEO BIOTROP
摘要：Normal 0 false false false MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} The objective of this study was to obtain the viability and plasma membrane integrity of the spotted buffalo epididymal sperm after addition of dextrose into Andromed Ò extender. Spermatozoa that have been collected from cauda epididymis were diluted with Andromed Ò extender as control (K) and Andromed Ò + 0.2% dextrose (P1) and Andromed Ò + 0.4% dextrose (P2) as treatments. The results showed that the quality of epididymal spermatozoa decreased during cryopreservation process. The percentage of motility after thawing in P1 (46%) and P2 (46.67%) were significantly higher (P Ò acted as an extra cellular cryoprotectant and could maintain the viability and membrane integrity of the spotted buffalo epididymal spermatozoa after thawing. Key words: epididymal sperm, cryopreservation, dextrose, spotted buffalo