文章基本信息

标题：ISOLATION AND CHARACTERIZATION OF METHANOTROPHIC BACTERIA FROM RICE FIELDS
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作者：Iman Rusmana ; ALINA AKHADIYA
期刊名称：BIOTROPIA
印刷版ISSN：1907-770X
出版年度：2009
卷号：16
期号：2
DOI：10.11598/btb.2009.16.2.53
语种：English
出版社：SEAMEO BIOTROP
摘要：Normal 0 false false false IN X-NONE X-NONE MicrosoftInternetExplorer4 st1\:*{behavior:url(#ieooui) } /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} Methane is a greenhouse gas and has potency to deplete ozone layer. Rice fields are a significantly sources of atmospheric methane. Chemical fertilizers application in rice fields can increase methane emission. Methanotrophic bacteria are unique in their ability to utilize methane as a sole carbon and energy source. This research was successfully isolating and characterizing methanotrophic bacteria from rice fields in Bogor and Sukabumi, West Java, Indonesia. Determination of methane oxidation activity using Gas Chromatography showed that all isolates performed methane oxidation activity. The highest methane oxidation activity was performed by BGM 9 isolate. And DNA amplification of BGM 9 genome was performed single band of mmo X in the size of 500 bp and three bands of pmo A in the size of 1000, 750 and 500 bp respectively