文章基本信息

标题：IN VITRO PROPAGATION OF ANGIOPTERIS EVECTA USING SPORES
本地全文：下载
作者：POONAM BHATIA ; DAMIEN CUPITT ; NANJAPPA ASHWATH 等
期刊名称：BIOTROPIA
印刷版ISSN：1907-770X
出版年度：2001
期号：16
DOI：10.11598/btb.2001.0.16.161
语种：English
出版社：SEAMEO BIOTROP
摘要：Techniques of establishing Angiopleris evecta plants in vitro were studied. Soaking of A. evecta spores in water for 24 hours markedly reduced spore contamination. Soaking of the spores in 1 -2 % of sodium hypochlorite for less than 5 minutes allowed satisfactory disinfestation without affecting spore viability. Lower concentration of minerals (1/4 MS), presence of charcoal in the medium and exposure of the spores to light were crucial for spore germination and gainetophytc development of A. evecta. Keywords: Angiopleris evecta I King fern / spore / MS medium / bleach / light / tissue culture / sporophyte / gamctophytc