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标题：HETEROLOGOUS EXPRESSION OF A CHITINASE GENE FROM AEROMONAS CAVIAEIN PSEUDOMONAS FLUORESCENS
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作者：ANTONIUS SUWANTO ; AMARILA MALIK ; BUDI TJAHJONO 等
期刊名称：BIOTROPIA
印刷版ISSN：1907-770X
出版年度：2003
期号：20
DOI：10.11598/btb.2003.0.20.182
语种：English
出版社：SEAMEO BIOTROP
摘要：A transcriptional fusion for an Aeromonas caviae chitinase gene was constructed under the control of a constitutive promoter of the kanaraycin resistance gene (PKmR ). The construct was inserted into a medium copy number broad host range plasmid vector to yield recombinant plasmid pAM340, which harbored transcriptional fusion PKmR - chi. Another transcriptional fusion, Ptac-chi, in a recombinant plasmid pAM630, was conducted as comparison. Triparental mating of E. coli carrying the recombinant plasmids with Pseudomotws fluorescens 5100, a phyllosphere bacterium, was performed. Pseudomonas fluorescens 5100 exconjugants were examined for constitutive expression of chitinase employing a spectrophotometric assay; they showed stronger chitin degradation activity than Escherichia coli transformants. Using a fungal antagonism plate assay, this chitinolytic P. fluorescens, however, could not inhibit selected phytopathogenic fungi. Keywords: Aeromonas caviae/ chitinase gene/transcriptional fusion/PKm'V Vtac-chilPseudomonas fluorescens