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标题：A SPECIES-SPECIFIC PCR ASSAY BASED ON THE INTERNAL TRANSCRIBED SPACER (ITS) REGIONS FOR IDENTIFICATION OF MYCOSPHAERELLA EUMUSAE, M. FIJIENSIS AND M. MUSICOLA ON BANANA
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作者：Iman Hidayat
期刊名称：BIOTROPIA
印刷版ISSN：1907-770X
出版年度：2012
卷号：19
期号：1
DOI：10.11598/btb.2012.19.1.232
语种：English
出版社：SEAMEO BIOTROP
摘要：Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} A study on development of a rapid PCR-based detection method based on ITS region of M. eumusae, M. fijiensis, and M. musicola on banana was carried out. The main objecive of this study was to develop a fast and species-specific PCR-based detection method for the presence of Mycosphaerella species on banana. The methods include collection of specimens, morphological identification supported by molecular phylogenetic analysis, RFLP analysis, species-specific primers development, and validation. Two species of Mycosphaerella , namely, M. fijiensis and M. musicola , and one unidentified Pseudocercospora species were found in Java island. Three restriction enzymes used in the RFLP analysis, viz, Alu I, Hae III, and Taq I were capable to discriminate M. eumusae , M. fijiensis , and M. musicola . Two species-specific primer pairs, viz, MfijF/MfijR and MmusF/MmusR have been successfully developed to detect for the presence of M. fijiensis and M. musicola , respectively.