文章基本信息

标题：Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing
本地全文：下载
作者：Estelle Silvia Kilias ; Ilka Peeken ; Katja Metfies 等
期刊名称：Polar Research
印刷版ISSN：1751-8369
出版年度：2014
卷号：33
期号：0
DOI：10.3402/polar.v33.23466
语种：English
出版社：Co-Action Publishing
摘要：Protists in the central Arctic Ocean are adapted to the harsh environmental conditions of its various habitats. During the Polarstern cruise ARK-XXVI/3 in 2011, at one sea-ice station, large aggregates accumulated at the bottom of the melt ponds. In this study, the protist assemblages of the bottom layer of the sea-ice and melt-pond aggregate were investigated using flow cytometry and 454-pyrosequencing. The objective is to provide a first molecular overview of protist biodiversity in these habitats and to consider the overlaps and/or differences in the community compositions. Results of flow cytometry pointed to a cell size distribution that was dominated by 3–10 µm nanoflagellates. The phylogenetic classification of all sequences was conducted at a high taxonomic level, while a selection of abundant (≥1% of total reads) sequences was further classified at a lower level. At a high taxonomic level, both habitats showed very similar community structures, dominated by chrysophytes and chlorophytes. At a lower taxonomic level, dissimilarities in the diversity of both groups were encountered in the abundant biosphere. While sea-ice chlorophytes and chrysophytes were dominated by Chlamydomonas/Chloromonas spp. and Ochromonas spp., the melt-pond aggregate was dominated by Carteria sp., Ochromonas spp. and Dinobryon faciluiferum. We suppose that the similarities in richness and community structure are a consequence of melt-pond freshwater seeping through porous sea ice in late summer. Differences in the abundant biosphere nevertheless indicate that environmental conditions in both habitats vary enough to select for different dominant species.Keywords: 18S rDNA; Arctic Ocean; biodiversity; next-generation sequencing; flow cytometryTo access the supplementary material for this article, please see supplementary files in the column to the right (under Article Tools)(Published: 5 November 2014)Citation: Polar Research 2014, 33, 23466, http://dx.doi.org/10.3402/polar.v33.23466
关键词：biology;18S rDNA; Arctic Ocean; biodiversity; next-generation sequencing; flow cytometry