文章基本信息

标题：Low cost liquid medium for in vitro cultivation of Leishmania parasites in low-income countries
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作者：Geremew Tasew ; Amha Kebede ; Dawit Wolday 等
期刊名称：Global Health Action
印刷版ISSN：1654-9716
电子版ISSN：1654-9880
出版年度：2009
卷号：2
期号：0
DOI：10.3402/gha.v2i0.2046
语种：English
出版社：Taylor & Francis
摘要：Background: Prompt laboratory diagnosis and initiation of treatment are effective components of leishmaniasis control. Detection of Leishmania parasites by ex-vivo culture of lesion scrapings is considered a definitive diagnostic method preceding initiation of treatment. Objective: A pilot study to find alternative medium that could reduce the cost of culturing from patient lesions for diagnosing leishmaniasis. Method: GALF-1 medium was formulated in our lab from locally available inexpensive solutions and powders in the presence of urine from healthy individuals. Amastigote to promastigote transformation, recovery of parasites after cryopreservation, cost and mass cultivation was compared using the following media: GALF-1, RPMI 1640, and conventional Locke’s semi-solid medium (LSSM), a modifications of Novy-MacNeal-Nicolle culture media, which uses Locke’s solution as an overlay. Results: GALF-1 preparation was cheap and the components available in low-income countries such as Ethiopia. Preparation was simple, not requiring autoclaving and extra distilled water. GALF-1 was able to transform amastigotes from Ethiopian patients’ samples and could be used to cultivate promastigotes in large quantities. GALF-1 decreased Leishmania culture costs by ~80-95% compared to LSSM and RPMI 1640, respectively. Promastigotes cultured with GALF-1 could be cryopreserved in liquid nitrogen with comparable re-culture potential. Conclusion: Affordability of diagnostic assays is a key issue for endemic resource-poor countries and the possibility to cut the cost of the efficient culture method for diagnosis through the use of inexpensive, locally formulated reagents could improve the diagnosis of leishmaniasis in Ethiopia and in other low-income countries. Keywords: Leishmania culture; urine; liquid medium; low-income countries (Published: 22 October 2009) Citation: Global Health Action 2009. DOI: 10.3402/gha.v2i0.2046
关键词：Global Health;Developing countries, Health intervention;RL, 760-785