文章基本信息

标题：Susceptibility of Clostridium difficile Toxins A and B to Trypsin and Chymotrypsin
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作者：D. M. Lyerly ; P. E. Carrig ; T. D. Wilkins 等
期刊名称：Microbial Ecology in Health and Disease
印刷版ISSN：1651-2235
出版年度：1989
卷号：2
期号：3
DOI：10.3402/mehd.v2i3.7468
语种：English
出版社：Microbial Ecology in Health and Disease
摘要：Previous studies describing the inactivation of toxins A and B of Clostridium difficile by trypsin and chymotrypsin used impure toxin and protease preparations. We repeated these studies using highly purified toxin, and trypsin and chymotrypsin that were not contaminated with each other. Our findings show that toxin A is resistant to trypsin but not chymotrypsin and that toxin B is inactivated by both proteases. Neither toxin was activated by the proteases.Keywords: C. difficile; Toxin A; Toxin B; Proteases.