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标题：Experimental Colitis Induced by Dextran Sulphate in Normal and Germfree Mice
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作者：A.-C. Bylund-Fellenius ; E. Landström ; L.-G. Axelsson 等
期刊名称：Microbial Ecology in Health and Disease
印刷版ISSN：1651-2235
出版年度：1994
卷号：7
期号：4
DOI：10.3402/mehd.v7i4.8184
语种：English
出版社：Microbial Ecology in Health and Disease
摘要：Experimental colitis in mice, induced by administration of 2.5 or 5 per cent dextran sodium sulphate (DSS; Mwt=40 kDa, S=16 per cent) in the drinking water, was characterised and optimised. The degree of colitis was evaluated after 7.10 d of acute exposure (5 per cent), after continuous supply for 22, 27 and 35 d (2.5 per cent), and after two and three 7 d cycles with DSS (5 per cent) in water. The colitis was manifested by typical clinical symptoms such as weight loss, rectal bleeding and diarrhoea. Macroscopically a shortening of the colon was observed and histologically epithelial cell damage, crypt loss and infiltration of mononuclear cells was verified. A stable subacute colitis with maintained diarrhoea (wet/dry weight of the faeces) and shortening of the colon, but no mortality or gross rectal bleeding was induced within 3 wk either by continuous supply of 2.5 per cent or by two 7 d periods of exposure to 5 per cent DSS. The development of colitis in germfree animals excludes a critical role of the intestinal microbiota for the induction of colitis by DSS.Keywords - Inflammatory bowel diseases, Colitis, ulcerative, Intestinal microorganisms.