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标题：Conjugal Transfer of Plasmid DNA between Lactococcus lactis Strains and Distribution of Transconjugants in the Digestive Tract of Gnotobiotic Rats
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作者：J. Schlundt ; P. Saadbye ; B. Lohmann 等
期刊名称：Microbial Ecology in Health and Disease
印刷版ISSN：1651-2235
出版年度：1994
卷号：7
期号：2
DOI：10.3402/mehd.v7i2.8166
语种：English
出版社：Microbial Ecology in Health and Disease
摘要：The conjugational transfer of the plasmid pAMβ1 between two strains of Lactococcus lactis subsp. lactis was measured in the intestinal tract of four conventional and eight gnotobiotic rats. In the conventional rats both donor and recipient strains were eliminated and transconjugants were not observed. Germfree rats were dosed orally with the recipient strain and the donor strain carrying pAMβ1. In faecal samples, transconjugants were detected within the first few days after dosing, and the transconjugants established a stable population at a level of 103—105 less than the recipient strain. In one group of animals the donor strain was eliminated from the intestine shortly after introduction, but still the transconjugants colonised the intestine. Samples from duodenum, jejunum, caecum and colon were cultured 2 or 5 wks after dosing with the recipient strain. The concentration of transconjugants was approximately 104c.f.u./g throughout the intestine, whereas the concentration of recipients increased from 104−105 c.f.u./g in jejunum to 108−109 c.f.u./g in caecum and colon. The plasmid pAMβ1 seems to have conveyed to the recipient a competitive advantage in the small intestine but not in the large intestine.Keywords - Lactococcus lactis, Gnotobiotic rat, Conjugation, Intestinal tract.