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标题：Colonisation of Germ-free Mice by Segmented Filamentous Bacteria after Oral Administration of Various Murine Intestinal Wall Preparations
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作者：H. L. B. M. Klaasen ; J. P. Koopman ; M. E. Van Den Brink 等
期刊名称：Microbial Ecology in Health and Disease
印刷版ISSN：1651-2235
出版年度：1990
卷号：3
期号：5
DOI：10.3402/mehd.v3i5.7550
语种：English
出版社：Microbial Ecology in Health and Disease
摘要：Intestinal suspensions containing segmented filamentous bacteria (SFBs) were given orally to germ-free mice in an attempt to pave the way for the production of mice mono-associated with SFBs. The effects of dilution, sonication and chloroform/ethanol treatment of intestinal suspensions on SFB colonisation were investigated. Colonisation density of SFBs in the germ-free mice was dependent on the number of SFBs in the administered suspension. Sonication of suspensions had no effect. Chloroform/ethanol treatment of suspensions resulted in the production of di-associated mice, containing both SFBs and a non-characterised clostridium in their small intestine. It is suggested that, through specific elimination of the clostridium, these di-associated animals may be used to produce mice containing SFBs alone.Keywords: Colonisation; Intestinal bacteria; Mouse; Segmented filamentous bacteria.