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标题：Butyrate and propionate production from D-mannitol in the large intestine of pig and rat
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作者：Makiko Maekawa ; Kazunari Ushida ; Seiko Hoshi 等
期刊名称：Microbial Ecology in Health and Disease
印刷版ISSN：1651-2235
出版年度：2005
卷号：17
期号：3
DOI：10.3402/mehd.v17i3.7781
语种：English
出版社：Microbial Ecology in Health and Disease
摘要：Fermentation of D-mannitol and the bacteria responsible for it were analyzed by 13C-NMR spectroscopy and amplified ribosomal restriction analyses (ARDRA) on intestinal bacteria in pig cecal digesta and in a rat in vivo feeding trial. [1-13C]- Mannitol was fermented by pig cecal digesta mainly to [2-13C]-propionate, [3-13C]-propionate, [2-13C]-butyrate, and [4-13C]-butyrate. Dietary D-mannitol at a 5% level induced significantly higher cecal butyrate concentration in the rat model. The evolution of rat fecal bacteria due to feeding with D-mannitol suggested the involvement of a lactate-utilizing butyrate producer, Clostridium indolis , in the stimulation of butyrate production. Rat intestinal microbiota is often difficult to extrapolate to that of humans, but the stimulation of the lactate-utilizing butyrate producers and propionate producers was suggested by the 13C-NMR spectroscopy of the end products of pig cecal fermentation and ARDRA on rat fecal bacteria. These results suggest the potential of D-mannitol as a prebiotic, which modifies large intestinal fermentation to produce more butyrate and propionate.Key words: D-Mannitol, 13C-NMR, butyrate, propionate, large intestine