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标题：Modeling the survivability of Salmonella typhimurium in the chicken cecae using an anaerobic continuous-culture of chicken cecal bacteria
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作者：Robin C. Anderson ; Donald E. Corrier ; Roger B. Harvey 等
期刊名称：Microbial Ecology in Health and Disease
印刷版ISSN：1651-2235
出版年度：2000
卷号：12
期号：1
DOI：10.3402/mehd.v12i1.8043
语种：English
出版社：Microbial Ecology in Health and Disease
摘要：An anaerobic continuous-culture containing 29-different bacterial isolates of normal avian microflora obtained from the cecae of adult chickens was used to model the survivability of Salmonella typhimurium in the avian cecae. When the continuous-culture was challenged with S. typhimurium at concentrations between 101-105 log10 CFU the pathogen was unable to survive within the culture. When challenged with 106 CFU S. typhimurium, the number of Salmonella CFU decreased to 102 within 5-days, however S. typhimurium was not cleared from the culture and remained at a concentration of 102 for 65-days at which time the fermentation was terminated. When chicks were provided the continuous-culture on day-of-hatch, then challenged with S. typhimurium two days later with up to 104 CFU Salmonella, no cecal colonization was observed 5-days after challenge. However chicks challenged with 106 CFU Salmonella averaged 102 S. typhimurium in cecal contents five days later. Chicks provided the continuous-culture on day-of-hatch and challenged with 102 or 104 CFU Salmonella had significantly (P<0.05) fewer cecal Salmonella compared to untreated chicks. Salmonella challenge did not effect chick cecal or continuous-culture fermentation parameters and fermentation parameters within the continuous-culture and chick cecae were similar. Based upon similar fermentation parameters and Salmonella colonization levels it appears that this anaerobic continuousculture of cecal bacteria is an excellent model of the cecae of chickens, and may serve as a valuable tool that can be used to better understand the interactions between Salmonella and normal avian flora in the cecae, which is the primary reservoir of Salmonella in commercial poultry.Keywords: Salmonella, continuous-culture, chicks, model.