文章基本信息

标题：Molecular Methods for the Analysis of Gut Microbiota
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作者：Pawel Namsolleck ; Ralph Thiel ; Paul Lawson 等
期刊名称：Microbial Ecology in Health and Disease
印刷版ISSN：1651-2235
出版年度：2004
卷号：16
期号：2-3
DOI：10.3402/mehd.v16i2-3.7939
语种：English
出版社：Microbial Ecology in Health and Disease
摘要：This review focuses on methodological approaches used to study the composition of human faecal microbiota. Gene sequencing is the most accurate tool for revealing the phylogenetic relationships between bacteria. The main application of fluorescence in situ hybridization (FISH) in both microscopy and flow cytometry is to enumerate faecal bacteria. While flow cytometry is a very fast method, FISH microscopy still has a considerably lower detection limit.Key words: DGGE, TGGE, dot blot, FISH, flow cytometry, gut ecology, in situ gene expression, micro-array, sequencing.