文章基本信息

标题：Nucleic Acid Probes in Microbiology
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作者：Neeraja Venkateswaran ; K.S. Venkateswaran
期刊名称：Defence Science Journal
印刷版ISSN：0976-464X
出版年度：2013
卷号：41
期号：4
页码：335-356
DOI：10.14429/dsj.41.4436
语种：English
出版社：Defence Scientific Information & Documentation Centre
摘要：Single-stranded nucleic acid molecules can interact under certain conditions with other nucleic acids in the regions of base complementarily to form double-stranded hybrid molecules. From measurement of kinetics of the hybridisation and the extent of hybrid formation, information on nucleotide sequence heterogeneity of nucleic acid fractions, sequence properties of purified classes of molecules or the degree of homology between different samples of nucleic acids can be obtained. A specific deoxyribonucleic acid or ribonucleic acid sequence with an identifiable marker can be used as a nucleic acid probe. This paper reviews the use of nucleic acid probes for classifying the microorganisms and their detection in clinical or environmental samples.
关键词：Diagnosis ;DNA ;RNA ;Hybridisation ;Deoxyribonucleic acid ;Ribonucleic acid