During a survey of polypores in the municipality of São Francisco de Paula, Rio Grande do Sul State, Brazil, 20 pileate species previously unregistered for the area were found and identified. Antrodia malicola, Coltricia aff. duportii, and Microporellus brasiliensis are new records for Rio Grande do Sul State. Comments on the 20 newly recorded species and an identification key for the studied area are presented.