Quality of life (QOL) has become a central issue of concern in peoples’ lives and the research on this topic has largely increased in the past decades. However, some studies on QOL only adopted objective indicators and QOL research is still very scarce in some fields of study such as tourism. Additionally, in tourism, the majority of the studies on QOL focus on assessing the QOL of residents of destinations and neglect segmentation based on QOL as a tool for market segmentation. The aim of this study is to overcome some previously referred to limitations and, specifically: (i) to measure visitors’ perceptions of tourism impact on their QOL by adopting subjective indicators; and (ii) to segment visitors based on perceptions of tourism impact on their QOL. Study results reveal that tourism is likely to have a more positive impact on the psychological and social domains of QOL and that QOL is a good basis for segmenting the tourism market. Visitors perceiving more positive tourism impacts in their QOL differ from others on several features such as motivations to travel, travel group, interaction with local residents and satisfaction with the trip.