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  • 标题:Identification of duplicates of cassava accessions sampled on the North Region of Brazil using microsatellite markers
  • 本地全文:下载
  • 作者:Moura, Elisa Ferreira ; Farias Neto, João Tomé de ; Sampaio, José Edson
  • 期刊名称:Acta Amazonica
  • 印刷版ISSN:0044-5967
  • 出版年度:2013
  • 卷号:43
  • 期号:4
  • 页码:461-467
  • DOI:10.1590/S0044-59672013000400008
  • 语种:English
  • 出版社:Instituto Nacional de Pesquisas da Amazônia
  • 摘要:

    Duplicates are common in germplasm banks and their identification is needed to facilitate germplasm bank management and to reduce maintenance costs. The aim of this work was to identify duplicates of cassava from a germplasm bank in Eastern Amazon, which had been previously characterized both morphological and agronomically. In order to be genotyped with 15 microsatellite loci, 36 accessions were selected. These accessions were classified into 13 groups of similar morpho-agronomical characteristics. All loci were polymorphic, and 75 alleles were identified, with an average of five alleles per loci and H E = 0.66. There were determined 34 pairs of genotypes with identical multiloci profiles and the probability of genetic identity was 1.1x10-12 with probability of exclusion of 99.9999%. Among these duplicates, there are accessions sampled on different years and places, but with different names and accessions with the same name sampled in different places and years. The study identified genotypes that are grown in different places and that have been maintained over the years by local farmers.

  • 关键词:Manihot esculenta;germoplasma;marcadores moleculares
  • 其他关键词:Manihot esculenta;germplasm;molecular markers
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