摘要:The optimization and the characterization of a new amperometric biosensor based on acetylcholinestrase (AChE), immobilized on a graphite electrode modified with Pt-nanoparticles (PtNP), are reported. The G/PtNP-AChE biosensor was used for heavy metals detection. The degree of inhibition (%I), the kinetic constants of the inhibition process, as well as the influence of the PtNP presence on these parameters were estimated.