文章基本信息

标题：Characterization of probiotic properties of Lactobacillus strains
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作者：Mathieu Millette ; François-Marie Luquet ; Marcia Teresa Ruiz 等
期刊名称：Dairy Science & Technology
印刷版ISSN：1958-5586
电子版ISSN：1958-5594
出版年度：2008
卷号：88
期号：6
页码：695-705
DOI：10.1051/dst:2008018
语种：English
出版社：EDP Sciences
摘要：The objectives of this study were to demonstrate the gastro-intestinal tolerance and the capacity to modulate the intestinal microbiota of some Lactobacillus acidophilus, L. casei and L. rhamnosus strains with high probiotic potential. L. acidophilus and L. casei mixture that is used to produce probiotic fermented milk has been evaluated for its acid resistance and bile salts tolerance and compared to other lactic acid bacteria (LAB). The commercial culture and the three strains of L. rhamnosus exhibited complete tolerance to acid for pH $\geq $ 2.5. The minimal inhibitory concentration of the bile salts mixture was 50 g$\cdot$L$^{-1}$ for all bacteria. The impact of the ingestion of the novel probiotic on the fecal microbiota was evaluated in vivo using healthy C57Bl/6 mice. Fecal samples were analyzed for the microbiota enumeration using selective plating. Fecal analysis showed an increase of total culturable LAB and a decrease in Staphylococcus spp. population in the LAB-treated mice indicating that these cultures could improve the intestinal health. Also, reduction in fecal Enterobacteriaceae was noticed following mice gavage with L. rhamnosus ATCC 9595 while a higher enumeration was measured for L. rhamnosus RW-9595M, an exopolysaccharide-overproducing mutant. These contradictory results were discussed.
关键词：probiotic;Lactobacillus;antimicrobial;gastrointestinal