文章基本信息

标题：Freezing stallion semen in INRA96$^{\circledR}$-based extender improves fertility rates in comparison with INRA82
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作者：Elodie Pillet ; Florence Batellier ; Guy Duchamp 等
期刊名称：Dairy Science & Technology
印刷版ISSN：1958-5586
电子版ISSN：1958-5594
出版年度：2008
卷号：88
期号：2
页码：257-265
DOI：10.1051/dst:2008002
语种：English
出版社：EDP Sciences
摘要：The chemical composition of freezing extenders plays a major role in sperm cell survival during cryopreservation. In this study we compared two extenders for freezing stallion semen: INRA82 extender (as a control) versus INRA96$^{\circledR}$ extender, both supplemented with egg yolk and glycerol. INRA82 contains milk, whereas INRA96$^{\circledR}$ is a chemically-defined extender developed for fresh semen storage at 4 $^{\circ}$C or 15 $^{\circ}$C. Semen from 3 stallions (7 ejaculates per stallion) was frozen in both extenders. In vitro analyses of post-thaw motility of sperm cells (computer-assisted analysis) and of membrane integrity (flow cytometry analysis) were performed. Then a fertility trial was conducted. Inseminations were conducted in a total of 84 mare cycles. INRA96$^{\circledR}$ extender supplemented with egg yolk and glycerol significantly improved per-cycle pregnancy rates compared with INRA82 (71% versus 40%, $p~$<$~$0.01). In agreement with these fertility results, membrane integrity was better preserved in INRA96$^{\circledR}$ than in INRA82. In contrast, motility parameters were significantly higher in INRA82 than in INRA96$^{\circledR}$. Further research is needed to understand how INRA96$^{\circledR}$ components protect sperm cells during the cryopreservation process and highly increase their fertility potential compared with INRA82 components.
关键词：cryopreservation;semen;INRA96$^{\circledR}$ extender;fertility;stallion