文章基本信息

标题：Identification of mono- or poly-specific monoclonal antibody to Porphyromonas gingivalis heat-shock protein 60
本地全文：下载
作者：Choi, Jeomil ; Lee, Sang-Yull ; Kim, Koanhoi 等
期刊名称：The Journal of the Korean Academy of Periodontology
印刷版ISSN：0250-3352
出版年度：2011
卷号：41
期号：2
页码：54-59
DOI：10.5051/jpis.2011.41.2.54
语种：English
出版社：Korean Academy of Periodontology
摘要：Purpose
The aim of this study was to define the immunoreactive specificity of Porphyromonas gingivalis ( P. gingivalis ) heat shock protein (HSP) 60 in periodontitis and atherosclerosis.
Methods
In an attempt to define the cross-reactive bacterial heat-shock protein with human self-antigen at molecular level, we have introduced a novel strategy for cloning hybridoma producing anti- P. gingivalis HSP 60 which is polyreactive to bacterial HSPs or to the human homolog.
Results
Five cross-reactive clones were obtained which recognized the #19 peptide (TLVVNRLRGSLKICAVKAPG) among 37 synthetic peptides (20-mer, 5 amino acids overlapping) spanning the whole molecule of P. gingivalis HSP 60. We have also established three anti- P. gingivalis HSP 60 monoclonal antibodies demonstrating mono-specificity. These clones recognized the #29 peptide (TVPGGGTTYIRAIAALEGLK).
Conclusions
Peptide #19 and #29 of P. gingivalis HSP 60 might be important immunoreactive epitopes in the immunopathogenic mechanism of bacterial antigen-triggered autoimmune diseases.
关键词：Antibodies; periodontitis; Porphyromonas gingivalis