文章基本信息

标题：The biologic effect of fibrin-binding synthetic oligopeptide on periodontal ligament cells
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作者：Lee, Ju-A ; Ku, Young ; Park, Yoon-Jeong 等
期刊名称：The Journal of the Korean Academy of Periodontology
印刷版ISSN：0250-3352
出版年度：2009
卷号：39
期号：1
页码：45-52
DOI：10.5051/jkape.2009.39.1.45
语种：Korean
出版社：Korean Academy of Periodontology
摘要：Purpose
Fibronectin(FN), one of the major components of ECM, mediates wide variety of cellular interactions including cell adhesion, migration, proliferation and differentiation. In this study, we used synthetic peptides based on fibrin binding sites of amino-terminal of FN and evaluated their biologic effects on periodontal ligament(PDL) cells.
Materials and methods
PDL cells were cultured on synthetic oligopeptides coated dishes and examined for cell adhesion, proliferation via confocal microscope. For detection of ERK1/2, cells were plated and Western blot analysis was performed.
Results
PDL cells on synthetic oligopeptide coated dishes showed enhanced cell adhesion and proliferation. Western blot analysis revealed increased level of ERK1/2 phosphorylation in cells plated on FN fragment containing fibrin-binding domain(FF1 and FF5) coated dishes.
Conclusion
These results reveals that FN fragment containing fibrin-binding domain possess an enhanced biologic effect of PDL ligament cells.
关键词：Fibronectin; Periodontal ligament cells; fibrin-binding domain; ERK1/2