文章基本信息

标题：Induction of IL-8 and reactive oxygen species in periodontal ligament cells by Aggregatibacter actinomycetemcomitans
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作者：Lee, Yang-Sin ; Park, Hong Gyu ; Kim, Sung Whan 等
期刊名称：The Journal of the Korean Academy of Periodontology
印刷版ISSN：0250-3352
出版年度：2009
卷号：39
期号：3
页码：331-337
DOI：10.5051/jkape.2009.39.3.331
语种：Korean
出版社：Korean Academy of Periodontology
摘要：Purpose
Interleukin (IL)-8 is one of pro-inflammatory cytokines. Reactive oxygen species (ROS) are reduced metabolites of O₂. Aggregatibacter actinomycetemcomitans is one of representative periodontopathogens. To investigate the role of A. actinomycetemcomitans in IL-8 expression of periodontal ligament (PDL) cells, we estimated the production of IL-8 and ROS in A. actinomycetemcomitans treated PDL cells.
Methods
The IL-8 production was determined by enzyme-linked immunosorbent assay. The ROS production was estimated using H₂DCFDA and FACS.
Results
A. actinomycetemcomitans increased the production of IL-8 and ROS at 10, 100, and 500 multiplicity of infection. N-acetylcysteine, an antioxidant of ROS, down-regulated the production of IL-8 induced by A. actinomycetemcomitans .
Conclusions
These results suggest that A. actinomycetemcomitans induces IL-8 production and ROS may act as a mediator in this process.
关键词：Aggregatibacter actinomycetemcomitans; Interleukin-8; Periodontal ligament; Reactive Oxygen Species