文章基本信息

标题：Effect of Dipyridamole on the Reactive Oxygen Species and Oxidative Stress in Trabecular Meshwork Cells
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作者：Lee, Keun Woo ; Kim, Jae Woo
期刊名称：Journal of the Korean Ophthalmological Society
印刷版ISSN：0378-6471
出版年度：2013
卷号：54
期号：3
页码：496-501
DOI：10.3341/jkos.2013.54.3.496
语种：Korean
出版社：The Korean Ophthalmological Society
摘要：Purpose
To investigate the effects of dipyridamole (DPD) on the production of reactive oxygen species (ROS) and oxidative stress in cultured human trabecular meshwork cells (HTMC).
Methods
Antioxidant activity of DPD was determined by DPPH assay. Primarily cultured HTMC were exposed to 0, 20, and 50 µm DPD using serum-deprived media. The effect of DPD on the production of ROS was assessed with the DCHFDA assay. The effect of DPD on the t-butyl hydroperoxide (tBHP)-induced oxidative stress was assessed with resazurin assay.
Results
DPD showed significant antioxidant activity. DPD significantly decreased the production of ROS ( p < 0.05) and improved cellular activity significantly after treatment with t-BHP ( p < 0.05). DPD did not affect the generation of nitric oxides.
Conclusions
DPD suppressed the formation of ROS and possessed cytoprotective activity against the oxidative stress in HTMC.
关键词：antioxidant; Dipyridamole; Reactive Oxygen Species; Trabecular meshwork cells