To compare central corneal thickness (CCT) as measured by dual rotating Scheimpflug camera (Galilei), anterior segment optical coherence tomography (AS-OCT), and ultrasound pachymetry (USP).

The measurements of CCT using a dual rotating Scheimpflug camera, AS-OCT, and USP in 40 eyes of 20 healthy subjects were compared.

The average measurements of CCT by dual rotating Scheimpflug camera, AS-OCT, and USP were 538.10 ± 31.36 µm, 536.20 ± 31.21 µm, and 541.93 ± 34.93 µm, respectively. The CCT measurement by USP was statistically significantly thicker than by the dual rotating Scheimpflug camera and AS-OCT ( p = 0.017, p = 0.001, respectively). There was no significant difference between the dual rotating Scheimpflug camera and AS-OCT ( p = 0.054). A significant linear correlation was observed between the dual rotating Scheimpflug camera, the AS-OCT, and the USP (r > 0.900, p < 0.001).

The results of the 3 methods have significant correlation with each other, but the measurement by USP was significantly thicker than the dual rotating Scheimpflug camera and AS-OCT. Therefore, CCT should be interpreted in the context of the instrument used.