文章基本信息

标题：Effect of Hydrogen Peroxide-induced Oxidative Stress on the Senescence of Trabecular Meshwork Cells
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作者：Kim, Jae Woo ; Kim, Sin Hoo ; Lee, Jae Hyung 等
期刊名称：Journal of the Korean Ophthalmological Society
印刷版ISSN：0378-6471
出版年度：2008
卷号：49
期号：10
页码：1665-1670
DOI：10.3341/jkos.2008.49.10.1665
语种：Korean
出版社：The Korean Ophthalmological Society
摘要：Purpose
To investigate the effects of oxidative stress on the senescence of trabecular meshwork (TM) cells and the effect of L-ascorbic acid (LAA) against oxidative stress-induced senescence.
Methods
Primary cultured human TM cells were exposed to 0.05 or 0.1 mM hydrogen peroxide for 30 minutes and incubated for 1 week with or without co-exposure of LAA. Cellular survival, nitrite production, and senescence were assessed with MTT, Griess, and SA-β-gal assays, respectively.
Results
Hydrogen peroxide decreased cellular survival and NO production accompanied increased cellular senescence. LAA did not prevent hydrogen peroxide-induced senescence.
Conclusions
Oxidative stress-induced senescence of TM cells may be related to the dysfunction of trabecular meshwork in glaucoma.
关键词：Ascorbic Acid; hydrogen peroxide; Oxidative stress; Senescence; Trabecular meshwork cells