文章基本信息

标题：Effect of Glucose on the Production of Reactive Oxygen Species in Retinal Pigment Epithelial Cells
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作者：Lee, Jae Hyung ; Kim, Jae Woo
期刊名称：Journal of the Korean Ophthalmological Society
印刷版ISSN：0378-6471
出版年度：2010
卷号：51
期号：2
页码：276-281
DOI：10.3341/jkos.2010.51.2.276
语种：Korean
出版社：The Korean Ophthalmological Society
摘要：Purpose
To investigate the effect of high glucose (HG) on the production of reactive oxygen species (ROS) in retinal pigment epithelial (RPE) cells.
Methods
ARPE-19 cells were exposed to low glucose (5 mM) and high glucose (HG, 25 mM) for three days. Additionally, 50 µM N-acetyl cysteine (NAC) and 100 µM L-arginine were co-exposed. Productions of nitric oxide (NO), ROS, and superoxide were determined by Griess assay, DCFH-DA assay, and modified cytochrome c assay, respectively.
Results
HG increased production of NO, ROS, and superoxide. HG-induced cells increased production of superoxide and ROS but were suppressed by NAC and L-arginine (substrate for NO production) as an antioxidant.
Conclusions
HG increased ROS production in RPE cells. Thus, HG may cause cellular dysfunction and damage by inducing oxidative stress in RPE cells.
关键词：High glucose; Oxidative stress; Reactive Oxygen Species; Retinal pigment epithelial cells