文章基本信息

标题：Role of Nitric Oxide in the Proliferative and Migratory Effect of Triamcinolone in RPE Cells
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作者：Kim, Jae Woo ; Lee, Jae Hyung ; Lee, Seung Hee 等
期刊名称：Journal of the Korean Ophthalmological Society
印刷版ISSN：0378-6471
出版年度：2010
卷号：51
期号：1
页码：120-125
DOI：10.3341/jkos.2010.51.1.120
语种：Korean
出版社：The Korean Ophthalmological Society
摘要：Purpose
To investigate the role of nitric oxide (NO) on the proliferative and migratory effects of triamcinolone acetonide (TA) in retinal pigment epithelial cells.
Methods
After exposure to 10 nM, 1 µM, or 100 µM TA for four days, with or without co-exposure of antioxidant N-acetylcyteine, the proliferation and nitrite production of ARPE19 cells were assessed with MTT and Griess assays, respectively. Additionally, a cell migration assay was performed.
Results
Cellular survival increased after exposure to TA at low concentration but decreased at high concentration. TA decreased the production of NO and cellular migration significantly, and these effects were abolished by N-acetylcysteine.
Conclusions
TA showed a biphasic response on the proliferation and decreased cellular migration in ARPE19 cells, which may be mediated by nitric oxide.
关键词：ARPE19; Migration; Nitric Oxide; Triamcinolone acetonide