Placement of the endotracheal tube is a potent airway irritant that may trigger bronchospasm in asthmatic patients. But bronchospasm has been reported in association with regional anesthesia, but no clear understanding of the mechanism involved. This case describes acute bronchospasm during spinal anesthesia in lower limb surgery. In this case, bronchospasm was relieved by intravenous injection of glycopyrrolate, not by β-agonist inhaler. We experienced repeated bronchospasm which was also very effectively treated by intravenous injection of glycopyrrolate.