文章基本信息

标题：Isolation and Characterization of Thermotolerant Alkaline Serine Protease of Bacillus sp. P-02
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作者：Rajeeva Gaur ; Soni Tiwari ; Alka Sharma 等
期刊名称：American Journal of Food Technology
印刷版ISSN：1557-4571
电子版ISSN：1557-458X
出版年度：2014
卷号：9
期号：5
页码：246-256
DOI：10.3923/ajft.2014.246.256
出版社：Academic Journals Inc., USA
摘要：Twelve bacterial strains isolated from the soils sample were screened for protease production on skimmed milk agar. Among them, strain P-02 was adjudged as the best protease producer and was identified as Bacillus sp. The isolate showed maximum protease production (3768 U mL^-1) in the presence of wheat bran and peptone at 60°C, pH 9.0 within 48 h of incubation. The enzyme activity was increased in the presence of Ca²⁺, Mg⁺² and Mn⁺² ions (0.1%). Protease activity was strongly inhibited in the presence of Hg and Fe ions. This isolate may be useful in several industrial applications owing to its thermo-tolerant and alkali-tolerant characteristics.