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标题：Agricultural residues for production of cellulase from Sporotrichum thermophile LAR5 and its application for saccharification of rice straw
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作者：Bajaj B. K. ; Sharma M. ; Rao R. S. 等
期刊名称：Journal of Materials and Environmental Science
印刷版ISSN：2028-2508
出版年度：2014
卷号：5
期号：5
页码：1454-1460
出版社：University of Mohammed Premier Oujda
摘要：Cellulases have got numerous industrial applications including saccharification of lignocellulosic biomass for environmentally-friendly production of bioethanol-biofuel and other platform chemicals. In the current stud y, fungal isolate Sporotrichum thermophile LAR5 successfully utilized low-cost agricultural residues as the substrates and produced considerable titre of cellulase. Wheat bran supported maximum cellulase production (2000 IU/L) and was followed by maize bran (1800 IU/L) and rice husk (1600 IU/L). Cellulase production was enhanced substantially b y peptone (7900 IU/L), mustard cake (7000 IU/L) and soybean meal (6000 IU /L) as co mpared to control (2000 IU/L); cotton cake and casein too supported higher enzyme production (3900 IU/L and 3800 IU/L, respectively) than control. Though optimum temperature for cellulase activity was 60-70 o C but significant activity was observed even at higher temperatures (80 -90 o C). Cellulase sho wed through thermostability at 50-60 o C for 30 min but stability deceased as the time and temperature increased further. Cellulase showed optimum pH of 5 but sufficient activity was observed over broad range of acidic and alkaline pH (3-10). Cellulase was thoroughly stable at pH 3 -6, however, towards higher alkaline pH stability gradually decreased. Furthermore, cellulase showed good saccharification ability on acid -pretreated rice straw, and could have potential for lignocellulosic biomass transformation.
关键词：Sporotrichum thermophile LAR5; Cellulase production; Agricultural residues; Rice straw; Saccharification