文章基本信息

标题：Using of EC Broth+Tryptophane and LST Broth+MUG Media for E. coli Counting
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作者：Hilal Beyhan Doğan ; A. Kadir Halkman
期刊名称：Gida : Journal of Food
印刷版ISSN：1300-3070
电子版ISSN：1309-6273
出版年度：1998
卷号：23
期号：6
出版社：Association of Food Technology, Turkey
摘要：In this research, to decrease the period for analysis of coliform, fecal coliform, E. coli analyzing according to TS/ISO 6063 by MPN method were investigated. A combined medium was developed by adding 0.1% tryptophane to EC Broth and 290 enterobacteria isolates (mostly coliforms) were analyzed for growth, gas and indol production at 44.5±0.5 ^oC. LST Broth+MUG was used as control medium for these tests in addition to fluorescence reaction so that if this medium may be an alternative to TS/ISO method. According to results, all 190 E. coli isolates gave equal result as expected in both culture media. It revealed that time period for analysis can be decreased to 3 days by using EC Broth+Tryptophane Medium instead of 6 days by the TSE/ISO 6063 method. No false reaction was obtained in LST Broth+MUG for 290 bacteria.