文章基本信息

标题：Determination of some Table and Wine Grape Varieties Grown in Turkey by Isoenzyme Identification
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作者：Gökhan Söylemezoğlu
期刊名称：Gida : Journal of Food
印刷版ISSN：1300-3070
电子版ISSN：1309-6273
出版年度：2001
卷号：26
期号：6
出版社：Association of Food Technology, Turkey
摘要：For both grape species and varieties, the morphological characters used for description are often strongly influenced by environmental factors and tissue ages, resulting in large variations in phenotype. For these reason, it was decided to use electrophoretic separation of isozymes from ripe berries of grape varieties. In this research, varieties were chosen because of their commercial importance and their availability at the Ankara University, Agricultural Faculty, Research and Experimental Vineyard. Enzyme banding patterns for 14 varieties of wine and table grapes were determined by polyacylamide gel electrophoresis. Enzymes were extracted from ripe berries of each variety. Enzyme bands were detected by developing the gels in a buffered solution that produced an insoluble dye at the site of enzyme activity. The varieties were assayed for Catechol Oxidase (CO) and Esterase (EST). Both enzymes were found quite useful for distinguishing varieties.