文章基本信息

标题：Cloning and Expressin of Citrate Permease Gene Lactococcus lactis subsp. lactisbiovar.diacetylactis MAD61
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作者：Mustafa Akçelik
期刊名称：Gida : Journal of Food
印刷版ISSN：1300-3070
电子版ISSN：1309-6273
出版年度：2001
卷号：26
期号：3
出版社：Association of Food Technology, Turkey
摘要：The citrate permease determinant (Cit-P) in Lactococcus lactis subsp. lactis biovar. diacetylactis MAD61 (L. diacetylactis) was demonstrated to be encoded by a 8.4 kb plasmid, pMC8, by protoplast induced plasmid curing experiments, The 1.9 kb Cit-P gene fragment was cloned into the Hindlll / Pstl site of plasmid pNZ9019. Resulting recombinant plasmid, pNZ9020, enabled Escherichia CO//E10-1 and citrate permease negative (Cit-P-) L diacetylactis MAD61 -22 to transport and utilize citrate, indicating expression of the citrate permease from strain MAD61 to interspecific and itraspecific hosts.