文章基本信息

标题：Identification of Different Meat Species Used in Sucuk Production by PCR Assay (in English)
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作者：Zülal Kesmen ; Hasan Yetim ; Fikrettin Şahin 等
期刊名称：Gida : Journal of Food
印刷版ISSN：1300-3070
电子版ISSN：1309-6273
出版年度：2010
卷号：35
期号：2
出版社：Association of Food Technology, Turkey
摘要：The PCR method has become a crucial tool for the identification of meats of different animal species in recent years and came into prominence as an alternative method that can replace the existing methods. In this study, a specific PCR method has been developed for identification of horse, donkey and porcine meats in a traditional, dry-fermented Turkish sausage, sucuk. 　For this purpose, sucuk samples were prepared from binary meat mixtures which were obtained by adding different amounts (0.0, 0.1, 0.5, 1.0 and 5.0%) of horse, donkey and porcine meats into either cattle or sheep meats. 　Oligonucleotide primers (producing fragments at a base pair (bp) length of 153, 145 and 227 respectively) that are specific to horse, donkey and porcine species were designed on the mitochondrial DNA. The results showed that each meat species could be identified at all the levels studied in the range of 0.1% to 5%, in all sucuk samples. In conclusion, it was suggested that the specific PCR method developed within the scope of this study could be used as a routine control method in food control laboratories for the identification of horse, donkey and porcine meats in the meat products.
关键词：PCR; meat species identification; sucuk; horse; donkey; porcine