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标题：D2-like receptor activation does not initiate a brain docosahexaenoic acid signal in unanesthetized rats
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作者：Ameer Y Taha ; Lisa Chang ; Mei Chen 等
期刊名称：BMC Neuroscience
印刷版ISSN：1471-2202
电子版ISSN：1471-2202
出版年度：2014
卷号：15
期号：1
页码：1
DOI：10.1186/1471-2202-15-113
语种：English
出版社：BioMed Central
摘要：Background The polyunsaturated fatty acid, docosahexaenoic acid (DHA), participates in neurotransmission involving activation of calcium-independent phospholipase A₂ (iPLA₂), which is coupled to muscarinic, cholinergic and serotonergic neuroreceptors. Drug induced activation of iPLA₂ can be measured in vivo with quantitative autoradiography using ¹⁴C-DHA as a probe. The present study used this approach to address whether a DHA signal is produced following dompaminergic (D)2-like receptor activation with quinpirole in rat brain. Unanesthetized rats were infused intravenously with ¹⁴C-DHA one minute after saline or quinpirole infusion, and serial blood samples were collected over a 20-minute period to obtain plasma. The animals were euthanized with sodium pentobarbital and their brains excised, coronally dissected and subjected to quantitative autoradiography to derive the regional incorporation coefficient, k*, a marker of DHA signaling. Plasma labeled and unlabeled unesterified DHA concentrations were measured. Results The incorporation coefficient (k*) for DHA did not differ significantly between quinpirole-treated and control rats in any of 81 identified brain regions. Plasma labeled DHA concentration over the 20-minute collection period (input function) and unlabeled unesterified DHA concentration did not differ significantly between the two groups. Conclusion These findings demonstrate that D2-like receptor initiated signaling does not involve DHA as a second messenger, and likely does not involve iPLA₂ activation.
关键词：Dopamine ; Quinpirole ; Neurotransmission ; Docosahexaenoic acid ; Release ; Signaling ; Metabolism ; Intracellular calcium ; Calcium-independent phospholipase A 2 (iPLA 2 ) ; Calcium-dependent phospholipase A 2 (cPLA 2 ) ; Arachidonic acid