文章基本信息

标题：Five-year tracking of Plasmodium falciparum allele frequencies in a holoendemic area with indistinct seasonal transitions
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作者：Hoseah M Akala ; Angela O Achieng ; Fredrick L Eyase 等
期刊名称：Journal of Multidisciplinary Healthcare
印刷版ISSN：1178-2390
电子版ISSN：1178-2390
出版年度：2014
卷号：7
页码：515-523
DOI：10.2147/JMDH.S67252
出版社：Dove Medical Press Ltd
摘要：Background: The renewed malaria eradication efforts require an understanding of the seasonal patterns of frequency of polymorphic variants in order to focus limited funds productively. Although cross-sectional studies in holoendemic areas spanning a single year could be useful in describing parasite genotype status at a given point, such information is inadequate in describing temporal trends in genotype polymorphisms. For Plasmodium falciparum isolates from Kisumu District Hospital, Plasmodium falciparum chloroquine-resistance transporter gene ( Pfcrt -K76T) and P. falciparum multidrug resistance gene 1 ( PfMDR1 -N86Y), were analyzed for polymorphisms and parasitemia changes in the 53 months from March 2008 to August 2012. Observations were compared with prevailing climatic factors, including humidity, rainfall, and temperature. Methods: Parasitemia (the percentage of infected red blood cells per total red blood cells) was established by microscopy for P. falciparum malaria-positive samples. P. falciparum DNA was extracted from whole blood using a Qiagen DNA Blood Mini Kit. Single nucleotide polymorphism identification at positions Pfcrt -K76T and PfMDR1 -N86Y was performed using real-time polymerase chain reaction and/or sequencing. Data on climatic variables were obtained from http://www.tutiempo.net/en/ . Results: A total of 895 field isolates from 2008 (n=169), 2009 (n=161), 2010 (n=216), 2011 (n=223), and 2012 (n=126) showed large variations in monthly frequency of PfMDR1 -N86Y and Pfcrt -K76T as the mutant genotypes decreased from 68.4%±15% and 38.1%±13% to 29.8%±18% and 13.3%±9%, respectively. The mean percentage of parasitemia was 2.61%±1.01% (coefficient of variation 115.86%; n=895). There was no correlation between genotype or parasitemia and climatic factors. Conclusion: This study shows variability in the frequency of Pfcrt -K76T and PfMDR1 -N86Y polymorphisms during the study period, bringing into focus the role of cross-sectional studies in describing temporal genotype trends. The lack of correlation between genotypes and climatic changes, especially precipitation, emphasizes the cost of investment in genotype change.
关键词：PfMDR1 -N86Y; Pfcrt -K76T; polymorphism; parasitemia