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标题：Differential Effect of Isooctane Doses on HaCaT and HeLa: A Multimodal Analysis
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作者：Lopamudra Das ; Sanmitra Basu ; Sanghamitra Sengupta 等
期刊名称：Advances in Toxicology
印刷版ISSN：2356-6906
出版年度：2014
卷号：2014
DOI：10.1155/2014/371497
出版社：Hindawi Publishing Corporation
摘要：A multimodal approach is effective in analyzing biological problems critically and thus also useful in assessing cytotoxicity under chemicals assaults. In this study effects of isooctane, an organic solvent and component of gasoline produced in petroleum industries, have been explored on normal (HaCaT) and cancerous (HeLa) epithelial cells. Besides morphological alterations, impacts on viability, prime molecular expressions, and bioelectrical properties on exposure to different doses of isooctane were noted. Scanning electron microscopy and viability assay demonstrated remarkable structural alterations and cell death, respectively, in HaCaT but not in HeLa. Transcriptomic and immunocytochemical studies on E-cadherin expression also elucidated pronounced toxic effects on HaCaT. Remarkable changes on the bioelectrical properties (e.g., impedance and phase angle) of the HaCaT, in contrast to HeLa, at different temporal points on isooctane exposure also indicated cytotoxic effects in the former. Hence this study illustrated cytotoxicity of isooctane on HaCaT multidimensionally which was evaded by HeLa.