文章基本信息

标题：In-Vivo Evaluation of Hexavalent Chromium Induced DNA Damage by Alkaline Comet Assay and Oxidative Stress in Catla catla
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作者：Arunachalam, Kantha Deivi ; Annamalai, Sathesh Kumar ; Kuruva, Jaya Krishna 等
期刊名称：American Journal of Environmental Sciences
印刷版ISSN：1553-345X
出版年度：2013
卷号：9
期号：6
页码：470-482
DOI：10.3844/ajessp.2013.470.482
出版社：Science Publications
摘要：In the present study, the acute toxicity of Chromium in fingerlings of Catla catla , an Indian major carp, was evaluated with renewal bioassay method. In vivo studies were designed to assess the extent of Micronucleus Assay, Comet Assay under the exposure of common heavy-metal compounds, namely, Chromium Nitrate, using Catla catla (2n = 20), as a test model. The laboratory acclimatized fishes were divided into four groups. Group I served as positive control and the other three as exposed groups for three different time durations of 7, 14 and 21 days and were subjected to uninterrupted sub lethal concentrations (50% of 96 h LC₅₀). The experiments were planned in such a way that fish from all the groups were sacrificed on the same day. The frequencies of micronuclei and bi-nuclei were evaluated comparatively in peripheral erythrocytes. As a result, it was observed that, the fishes and different tissues showed differential sensitivity to the heavy-metal treatment. A significant increase in the frequencies of micronucleated and binucleated cells and percentage increase in DNA tail (p Catla catla during sub lethal toxicity study was also calculated.
关键词：Chromium; Micronucleus; Catla catla; Cytotoxicity; Comet Assay