文章基本信息

标题：A DNA delivery system targeting dendritic cells for use in immunization against malaria: a rodent model
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作者：GB Keto ; SE West ; Microbiology, University of Bristol, Bristol, UK 等
期刊名称：Tanzania Journal of Health Research
印刷版ISSN：0856-6496
电子版ISSN：1821-9241
出版年度：2005
卷号：7
期号：3
页码：142-148
DOI：10.4314/thrb.v7i3.14251
语种：English
出版社：African Journals Online
摘要：DNA-based vaccination has emerged as a promising method of immunisation since the first demonstration of this technology. Improving the antibody responses is desirable for the protective efficacy and hence broad application of these vaccines. We examined the immunogenicity of a Plasmodium-based DNA vaccine that was targeted to antigen presenting cells by fusion to CTLA4. Fusion proteins comprising the extra-cellular domain of CTLA4, the hinge, CH2 and CH3 domains of human IgG1 and MSP-1 gene fragments were expressed in COS-7 cells. Three of the secreted proteins containing the mouse homologue of CTLA4 were shown to bind differently to the human B7-1 molecule expressed on THP-1 cells. Competition binding assays for two fusion proteins showed that binding was specific. When C57BL/6 mice were immunized with plasmids encoding the fusion proteins, antibodies against two denatured and one non-denatured MSP-1 gene fragments were successfully induced. The usefulness of this strategy in future studies of immunisaton against human malaria is discussed. Keywords : malaria, PbMSP-1, DNA vaccine, dendritic cells, rodent model Tanzania Health Research Bulletin Vol. 7(3) 2005: 142-148