文章基本信息

标题：Reactive Arrays of Colorimetric Sensors for Metabolite and Steroid Identification
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作者：Gary Batres ; Talia Jones ; Hannah Johnke 等
期刊名称：Journal of Sensor Technology
印刷版ISSN：2161-122X
电子版ISSN：2161-1238
出版年度：2014
卷号：4
期号：1
页码：1-6
DOI：10.4236/jst.2014.41001
出版社：Scientific Research Publishing
摘要：The work described herein examines a rapid mix-and-measure method called DETECHIP suitable for screening of steroids and metabolites. The addition of steroids and metabolites to reactive arrays of colorimetric sensors generated characteristic color “fingerprints” that were used to identify the analyte. A color analysis tool was used to identify the analyte pool that now includes biologically relevant analytes. The mix-and-measure arrays allowed the detection of disease metabolites, orotic acid and argininosuccinic acid; and the steroids androsterone, 1,4-androstadiene, testosterone, stanozolol, and estrone. The steroid 1,4-androstadiene was also detected by this method while dissolved in synthetic urine. Some of the steroids, such as androstadiene, stanozolol, and androsterone were co-dissolved with (2-hydroxypropyl)-β-cyclodextrin in order to increase solubility in aqueous buffered solutions. The colorimetric arrays do not intend to eliminate ELISA or mass spectroscopy based screening, but to possibly provide an alternative analytical detection method for steroids and metabolites.
关键词：Colorimetric Arrays; Sensors; Color Changes; Steroids; Metabolic Disease; Metabolites; Detection