摘要:The study focus was an examination of
the hydrothermal pretreatment method applied to the lignocellulosic substrate,
represented by the prairie cord grass, and comparison between different conditions
based on the yield of glucose after enzymatic hydrolysis. The treatment did not
involve any chemicals usage. Enzymatic hydrolysis was performed in order to
examine the amount of glucose which was released from pretreated materials. The
most efficient pretreatment conditions were at high temperature
and relatively short reaction time (210°C and 10 min), after which the
lignocellulose structure was the most available for enzymes actions which
resulted in a pretreatment conversion rate of 97%. Temperature had a significant
influence on glucose release during the hydrolysis, which was confirmed by the
Michaelis-Menten and kinetic models. Kinetic
models were used to fit the inhibitors and their conversion rates were related
to temperature.