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标题：Immunogenicity Characterization of Two Ancient Wheat α-Gliadin Peptides Related to Coeliac Disease
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作者：Armando Gregorini ; Mariastella Colomba ; H. Julia Ellis 等
期刊名称：Nutrients
电子版ISSN：2072-6643
出版年度：2009
卷号：1
期号：2
页码：276-290
DOI：10.3390/nu1020276
语种：English
出版社：MDPI Publishing
摘要：The immunogenic potential of α-gliadin protein from two ancient wheats was studied with reference to coeliac disease. To this aim we investigated Graziella Ra® and Kamut® (the latter is considered an ancient relative of modern durum wheat) in comparison to four durum wheat accessions (Senatore Cappelli, Flaminio, Grazia and Svevo). ELISA and Western Blot analyses - carried out by two monoclonal antibodies raised against the α-gliadin peptides p31-49 (LGQQQPFPQQPYPQPQPF) and p56-75 (LQLQPFPQPQLPYPQPQLPY) containing a core region (underlined) reported to be toxic for coeliac patients - always showed an antibody-antigen positive reaction. For all accessions, an α-gliadin gene has also been cloned and sequenced. Deduced amino acid sequences constantly showed the toxic motifs. In conclusion, we strongly recommend that coeliac patients should avoid consuming Graziella Ra® or Kamut®. In fact their α-gliadin not only is as toxic as one of the other wheat accessions, but also occurs in greater amount, which is in line with the higher level of proteins in ancient wheats when compared to modern varieties.
关键词：coeliac disease; ancient wheats; α-gliadin; toxic peptides; monoclonal antibodies; Triticum turgidum durum