文章基本信息

标题：Microstructured Block Copolymer Surfaces for Control of Microbe Adhesion and Aggregation
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作者：Ryan R. Hansen ; Katherine R. Shubert ; Jennifer L. Morrell-Falvey 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2014
卷号：4
期号：1
页码：63-75
DOI：10.3390/bios4010063
语种：English
出版社：MDPI Publishing
摘要：The attachment and arrangement of microbes onto a substrate is influenced by both the biochemical and physical surface properties. In this report, we develop lectin-functionalized substrates containing patterned, three-dimensional polymeric structures of varied shapes and densities and use these to investigate the effects of topology and spatial confinement on lectin-mediated microbe immobilization. Films of poly(glycidyl methacrylate)-block-4,4-dimethyl-2-vinylazlactone (PGMA-b-PVDMA) were patterned on silicon surfaces into line arrays or square grid patterns with 5 μm wide features and varied pitch. The patterned films had three-dimensional geometries with 900 nm film thickness. After surface functionalization with wheat germ agglutinin, the size of Pseudomonas fluorescens aggregates immobilized was dependent on the pattern dimensions. Films patterned as parallel lines or square grids with a pitch of 10 μm or less led to the immobilization of individual microbes with minimal formation of aggregates. Both geometries allowed for incremental increases in aggregate size distribution with each increase in pitch. These engineered surfaces combine spatial confinement with affinity-based capture to control the extent of microbe adhesion and aggregation, and can also be used as a platform to investigate intercellular interactions and biofilm formation in microbial populations of controlled sizes.
关键词：lectins; exopolysaccharide; affinity-based capture; cell adhesion; cell aggregation; block copolymers; biofilms