文章基本信息

标题：ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin
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作者：Zafar Hussain Ibupoto ; Syed Muhammad Usman Ali ; Kimleang Khun 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2011
卷号：1
期号：4
页码：153-163
DOI：10.3390/bios1040153
语种：English
出版社：MDPI Publishing
摘要：In this study, we have successfully demonstrated the fabrication of a biosensor based on well aligned single-crystal zinc oxide (ZnO) nanorods which were grown on gold coated glass substrate using a low temperature aqueous chemical growth (ACG) method. The ZnO nanorods were immobilized with penicillinase enzyme using the physical adsorption approach in combination with N-5-azido-2-nitrobenzoyloxysuccinimide (ANB-NOS) as cross linking molecules. The potentiometric response of the sensor configuration revealed good linearity over a large logarithmic concentration range from 100 µM to 100 mM. During the investigations, the proposed sensor showed a good stability with high sensitivity of ~121 mV/decade for sensing of penicillin. A quick electrochemical response of less than 5 s with a good selectivity, repeatability, reproducibility and a negligible response to common interferents such as Na1+, K1+, d-glucose, l-glucose, ascorbic acid, uric acid, urea, sucrose, lactose, glycine, penicilloic acid and cephalosporins, was observed.
关键词：zinc oxide nanorods; enzyme penicillinase; potentiometric nanosensors; N-5-azido-2-nitrobenzoyloxysuccinimide; nanodevices