文章基本信息

标题：Computational design of a pH-sensitive IgG binding protein
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作者：Eva-Maria Strauch ; Sarel J. Fleishman ; David Baker 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2014
卷号：111
期号：2
页码：675-680
DOI：10.1073/pnas.1313605111
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Computational design provides the opportunity to program protein-protein interactions for desired applications. We used de novo protein interface design to generate a pH-dependent Fc domain binding protein that buries immunoglobulin G (IgG) His-433. Using next-generation sequencing of naive and selected pools of a library of design variants, we generated a molecular footprint of the designed binding surface, confirming the binding mode and guiding further optimization of the balance between affinity and pH sensitivity. In biolayer interferometry experiments, the optimized design binds IgG with a Kd of [~]4 nM at pH 8.2, and approximately 500-fold more weakly at pH 5.5. The protein is extremely stable, heat-resistant and highly expressed in bacteria, and allows pH-based control of binding for IgG affinity purification and diagnostic devices.
关键词：computational interface design ; pH-sensitivity ; antibody purification