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标题：Serological and nested PCR survey to determine the occurrence of chlamydia infections in the Polish cattle population
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作者：Monika Szymańska-Czerwińska ; Krzysztof Niemczuk ; Monika Elżbieta Galińska 等
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2013
卷号：20
期号：4
页码：682-686
出版社：Institute of Agricultural Medicine in Lublin
摘要：Chlamydia spp. is an obligate intracellular agent that causes chlamydiosis in animals and humans. The aim of the presented study was to investigate the prevalence of Chlamydia infection in the Polish cattle population, both asymptomatic and having reproduc tive disorders. The study was performed on 4,475 serum samples collec ted from 16 Polish provinces at the turn of 2009 –2011. The samples (3,419 from asymptomatic cattle and 1,056 from cattle with reproduc tive disorders) were tested by complement fixation test (CFT). Moreover, 160 and 201 samples of biological materials from both groups of cattle, respectively, were tested by nested PCR. The results obtained for two tested groups were compared by χ2 (ch-squared) test, both individually for each region (province), and generally for the whole country. The CFT results showed that the seroprevalence of Chlamydia spp. infections in the asymptomatic cattle population was 4.15%, while in the cattle with reproductive disorders – 7.20%. There was a significant statistical difference between compared groups for whole country, but there were no significant differences for individual provinces. The results of PCR showed that Chlamydia spp. was present in both asymptomatic cattle and cattle having reproductive disorders. The nested PCR study confirmed the presence of Chlamydia abortus and Chlamydia suis in the tested samples. The presented study indicates that infec tions with Chlamydia spp. are present among Polish cattle, but the percentage of infected animals is not high
关键词：cattle; Chlamydia spp.; chlamydiosis; complement fixation test; PCR