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标题：Air samplings in a Campylobacter jejuni positive laying hen flock
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作者：Marwa Fawzy El Metwaly Ahmed ; Jochen Schulz ; Joerg Hartung 等
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2013
卷号：20
期号：1
页码：16-20
出版社：Institute of Agricultural Medicine in Lublin
摘要：The air in laying hen houses contains high concentrations of airborne bac teria. The numbers of these bacteria can be influenced by the efficiency of the chosen sampling method. In the presented study, AGI-30 Impingers and the Coriolis.μ air Sampler were compared in terms of their efficienc y in sampling aerobic mesophilic bac teria in a laying hen house. Measurements were conduc ted in a laying hen flock with high prevalences of C. jejuni in order to investigate if culturable cells of this organism can also be detected by the applied methods. Airborne dust was also analyzed for the presence of C. jejuni specific DNA to assess the possible occurrence of non-culturable C. jejuni in the hen house air. The numbers of mesophilic airborne bac teria ranged from 8 × 104– 2 × 106 CFU/m-3when sampled using AGI-30 Impingers, and from 2 × 105– 4 × 106 CFU/m-3when sampled using a Coriolis.μ air Sampler. The concentrations detec ted simultaneously by both devices correlated well (rPearson= 0.755), but the Coriolis.μ air Sampler showed a significantly higher sampling efficiency (p < 0.001). Although, the within flock prevalence of C. jejuni was high during the experiments (between 70-93%), neither of the air sampling methods could detect culturable C. jejuni from the air. However, C. jejuni specific DNA was detected in 15 out of 18 airborne dust samples by mapA PCR. Based on the results, it can be concluded that airborne culturable C. jejuni were not detec table, even with an efficient air sampler, because of their low concentration. Therefore, the risk of airborne infec tion to poultry workers on inhaling airborne C. jejuni seems negligible. Also, the transmission of culturable C. jejuni to neighboring farms by the airborne route is unlikely. Otherwise, the detec tion of airborne C. jejuni specific DNA suggests that non-culturable cells could appear in the hen house air, and in future it should be verified whether sampling stress of the air sampling methods could induce the non-culturable state
关键词：AGI-30 impinger; Coriolis.μ air Sampler; airborne bacteria; Campylobacter jejuni; laying hens