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标题：Borrelia burgdorferi sensu lato as activators of the complement system in in vitro model.
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作者：Małgorzata Tokarska-Rodak ; Maria Kozioł-Montewka ; Hanna Fota-Markowska 等
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2012
卷号：19
期号：4
页码：641-645
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction. The following parameters were analyzed: C5a, which is signi cant in classical and alternative pathways of the complement system activation, and factor H, the major func tion of which is to regulate the alternative pathway. Fac tor H, in the case of Borrelia infection, is combined by CRASPs proteins of spirochetes, and thus prevents C3b molecules from contact with the pathogen, opsonisation and lysis of bacteria.Material and Methods. The experimental material in the model for the presented work consisted of whole blood of healthy persons (without the presence of antibodies anti-Borrelia) and persons with clinical symptoms of Lyme disease, which was stimulated with three genospecies of spirochetes recognized as pathogenic in Poland and Europe: B. afzelii, B. burgdorferi s.s. and B. garinii.Results. The increase in the level of C5a in the experimental model after in vitro stimulation of whole blood with three genospecies Borrelia can be treated as an indicator of an e ective activation of the complement's cascade. The increase in level C5a in the plasma relies on the genospecies and the strongest is for B. garinii. The decrease in the level of fac tor H, observed af ter the incubation of whole blood with three genospecies Borrelia, shows that this parameter was included in the spirochetes' mechanisms acting against factors of the innate immunity system of a host, which prevents lysis of bacteria via the alternative pathway.Conclusions. The results obtained on the basis of the in vitro model can be analysed from the aspect of spirochetes' real contac t with a host's organism during the bite of infected ticks. Despite blocking of the alternative pathway, Borrelia initiate the activation cascade regardless of antibodies via the rst contac t of a host's organism with spirochetes, or in accordance with antibodies during the infection or subsequent contac t with bacteria
关键词：Complement system; Borrelia burgdorferi; Fac tor H; C5a